Development and validation of RP-HPLC method for the simultaneous estimation of rabeprazole sodium and lafutidine in combined dosage form

A simple, rapid, specific, accurate and precise RP-HPLC method was developed for the simultaneous estimation of Lafutidine Rabeprazole Sodium in combined dosage form. Hi Q sil C-8 Column (250 mm × 4.6 mm, 5 μm) Isocratic mode with mobile phase containing Acetonitrile : Phosphate Buffer (70:30 v/v) pH 7.3 used. The flow rate 1.0ml/min effluents were monitored at 230 nm. retention time found to be 3.658 min 5.408 respectively. Validation parameters such as linearity, precision, accuracy, limit detection(LOD), quantification (LOQ), robustness determined according International Conference on Harmonization (ICH) Q2R1 guidelines. detector response linear range 10-60 μg/ml 20-120 μg/ml. proposed successfully applied LAF RAB pharmaceutical